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Abd El-Tawab, A., Ammar, A., Hofy, F., Mohamed, S., Abubakr, H. (2017). Studies on Virulence Genes of Staphylococcus Aureus Isolated from Mastitic Cows. Benha Journal of Applied Sciences, 2(3), 89-93. doi: 10.21608/bjas.2017.164554
A.A. Abd El-Tawab; A.M. Ammar; F.I. Hofy; S.R. Mohamed; H.S. Abubakr. "Studies on Virulence Genes of Staphylococcus Aureus Isolated from Mastitic Cows". Benha Journal of Applied Sciences, 2, 3, 2017, 89-93. doi: 10.21608/bjas.2017.164554
Abd El-Tawab, A., Ammar, A., Hofy, F., Mohamed, S., Abubakr, H. (2017). 'Studies on Virulence Genes of Staphylococcus Aureus Isolated from Mastitic Cows', Benha Journal of Applied Sciences, 2(3), pp. 89-93. doi: 10.21608/bjas.2017.164554
Abd El-Tawab, A., Ammar, A., Hofy, F., Mohamed, S., Abubakr, H. Studies on Virulence Genes of Staphylococcus Aureus Isolated from Mastitic Cows. Benha Journal of Applied Sciences, 2017; 2(3): 89-93. doi: 10.21608/bjas.2017.164554

Studies on Virulence Genes of Staphylococcus Aureus Isolated from Mastitic Cows

Article 12, Volume 2, Issue 3, December 2017, Page 89-93  XML PDF (362.24 K)
Document Type: Original Research Papers
DOI: 10.21608/bjas.2017.164554
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Authors
A.A. Abd El-Tawab1; A.M. Ammar2; F.I. Hofy1; S.R. Mohamed3; H.S. Abubakr3
1Bacteriology, Immunology and Mycology Dept., Faculty of Veterinary Medicine, Benha Univ., Benha, Egypt
2Bacteriology,Immunology and Mycology Dept., Faculty of Veterinary Medicine, Benha Univ., Benha, Egypt
3Animal Health Research Institute(Dokki branch)
Abstract
Staphylococcus aureus is the most frequently bacterial pathogen causing clinical and subclinical mastitis in cattle. In this research the mastitic cases (103) were classified into clinical and subclinical cases .(47,56) respectively. All milk samples collected from infected cows were subjected to bacteriological examination and molecular characterization of some Staphylococcus aureus isolates. Staphylococcus aureus was isolated from clinical and subclinical mastitic cows in an incidence of (50%,17%)respectively . The application of multiplex PCR on some Staph.aureus isolates (8) was effectively in detection of Coa. SpA, ,TST,HIg, CLF, nucgenes by amplification at a single amplicon at (630bp, 226 bp,326bp, 937bp, 638bp, 395bp respectively).
Keywords
S.aureus; cows; mastitis; Pcr; virulence genes
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