Effect of Genetic Variations in BMP 4 Gene (Exon 2 Plus Part Of Intron 2) on Infertility in Egyptian Buffalo Cows

Baloza, S.H.; Abo-Salem, M.E.S.; Hemeda, S.A.

doi:10.21608/bjas.2017.167116

Effect of Genetic Variations in BMP 4 Gene (Exon 2 Plus Part Of Intron 2) on Infertility in Egyptian Buffalo Cows

Document Type : Original Research Papers

Authors

¹ Animal Wealth Development Dept., Faculty of Veterinary Medicine, Benha Univ. ,Egypt

² Toxicology and Forensic medicine Dept., Faculty of Veterinary Medicine, Benha Univ., Egypt

³ Animal Husbandry and Wealth Development Dept., Faculty of Veterinary Medicine, Alex. Univ., Egypt

10.21608/bjas.2017.167116

Abstract

Bone morphogenetic proteins (BMPs) are growth factors belonging to the transforming growth factor beta
(TGF-β) superfamily. BMPs are intra-ovarian factors expressed in mammalian ovaries by oocytes, granulosa and theca cells. The present study was designed to isolate and detect polymorphism in BMP 4 (exon 2 plus part of intron 2) gene associated with infertility in Egyptian buffalo cows using PCR-RFLP and nucleotide sequencing techniques. PCR amplification of DNA from 35 Egyptian water buffalo (5 normal fertile, 14 anestrum and 16 repeat breeders) with 482 bp expected size was purified and sequenced. There was no variation in the sequence of amplified region between fertile and infertile group. PCR product was digested using Taqα1 and Hinf1 restriction enzymes gave one type of the restriction banding pattern with no polymorphism. Concluded that BMP 4 is highly evolutionarily conserved gene.

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