Isolation and screening of cellulase producing bacteria isolated from soil

Abdel-Aziz, S.H.; Ibrahim, A.M.; Guirgis, A.A; Dawwam, G.E.; Elsababty, Z.E.

doi:10.21608/bjas.2021.188849

Isolation and screening of cellulase producing bacteria isolated from soil

Document Type : Original Research Papers

Authors

¹ Botany and Microbiology, Dept., Faculty of Science, Benha Univ., Benha, Egypt

² Microbial Biotechnology, Dept., Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Menoufia, Egypt

10.21608/bjas.2021.188849

Abstract

Cellulases are enzymes produced by many organisms and cellulose hydrolysis. They are well-known for their widely distributed industrial and medical uses. In this work, 84 bacterial strains were recovered from agar plates and 24 demonstrated hydrolyzing areas from carboxymethyl cellulose-containing agar dishes following Congo-Red staining. Among the 24 strains, three isolates Z7, Z9 and Z63 have shown increased activity of carboxymethyl cellulase (CMCase). It was shown that the three strains, Bacillus and Klebsiella, belonged to 2 separate genera. The isolates have been morphologically, physiologically, biochemically determined and validated by their 16S rRNA gene sequence. The 16S rRNA sequence of Z7, Z9 and Z63 has been submitted with GenBank under the accession codes (KT693283, KT693282 and KT693284). The sequences have been identified as Bacillus cereus strain Z7, Bacillus licheniformis strain Z9 and Klebsiella oxytoca strain Z63. This study gave appropriate information on the variety of rhizospheric isolated cellulose-degrading bacteria.

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