El Dougdoug, N., El Shahat, H., Amer, M., El Dougdoug, W., El Dougdoug, K. (2021). Action Mechanism of Synthesized Nitrogen Base Analogues that inhibit Tomato Mosaic tobamovirus. Benha Journal of Applied Sciences, 6(3), 269-278. doi: 10.21608/bjas.2021.188868
Noha.K. El Dougdoug; Hala.K. El Shahat; M.M. Amer; W.A. El Dougdoug; K.A. El Dougdoug. "Action Mechanism of Synthesized Nitrogen Base Analogues that inhibit Tomato Mosaic tobamovirus". Benha Journal of Applied Sciences, 6, 3, 2021, 269-278. doi: 10.21608/bjas.2021.188868
El Dougdoug, N., El Shahat, H., Amer, M., El Dougdoug, W., El Dougdoug, K. (2021). 'Action Mechanism of Synthesized Nitrogen Base Analogues that inhibit Tomato Mosaic tobamovirus', Benha Journal of Applied Sciences, 6(3), pp. 269-278. doi: 10.21608/bjas.2021.188868
El Dougdoug, N., El Shahat, H., Amer, M., El Dougdoug, W., El Dougdoug, K. Action Mechanism of Synthesized Nitrogen Base Analogues that inhibit Tomato Mosaic tobamovirus. Benha Journal of Applied Sciences, 2021; 6(3): 269-278. doi: 10.21608/bjas.2021.188868
Action Mechanism of Synthesized Nitrogen Base Analogues that inhibit Tomato Mosaic tobamovirus
1Microbiology, and Botany, Dept., Faculty of Science, Benha Univ., Benha, Egypt
2Chemistry, Dept., Faculty of Science, Benha Univ., Benha, Egypt
3Microbiology, Dept., Faculty of Agriculture, Ain Shams Univ, Cairo, Egypt
Abstract
Synthetic Nitrogen Base Analogues (SNBA) fused heterocyclic purine and pyrimidine derivatives are of great biological interest, especially as antiviral, antitumor and antimicrobial agents. The viruses are one of the risk factors for plants resulting in gigantic economic losses . Four SNBA compounds were assayed antiviral activity, plant cytotoxicity as well as determining their mode of action against Tomato mosaic tobamovirus (ToMV) by using the local lesion on Datura metel as well as infectivity assayed on tomato plants . The cytotoxicity of Ph-Oxanzolo and Oxazalone compounds at concentrations 15 and 20μg/mL showed chlorosis, mottling, necrosis, wilting, and curling at three to four days after spraying while Hippuric acid and Benzotriazolo compounds were safe on test plants and didn’t show any signs of toxicity on D.metel and tomato plants . Ph-Oxanzolon and Oxazalone had virucidal activity where showed high percentage of inhibition at simultaneous- ToMV inoculation at 5,10,15,20 ppm concentration and low percentage of inhibition at pre-and –post ToMV inoculation , as well as they showed high ToMV concentration and low percentage disease severity at 10 ppm concentration . While another Hippuric acid and Benzotriazol had affect the ToMV replication process , where showed weak inhibitory activity of inhibition at simultaneous - ToMV inoculation at 5,10,15,20 ppm concentration and low percentage of inhibition at pre-and –post ToMV inoculation , as well as they showed very low doubtful ToMV concentration and disease severity percentage at 15 ppm concentration .
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