Production and Optimization of Bioflocculant isolated from bacteria

Hassan, Mervat.G.; El Awady, M.E.; Mohamed, Sahar.S.; Elbanna, H.W.; Abdel-Monem, M.O.

doi:10.21608/bjas.2022.257798

Production and Optimization of Bioflocculant isolated from bacteria

Document Type : Original Research Papers

Authors

¹ Botany and Microbiology Department, Faculty of science, Benha University, 13518 Benha,Egypt

² Microbial Biotechnology Department, National Research Centre, El-Buhouth St. 33, Dokki-Cairo 12622, Egypt

10.21608/bjas.2022.257798

Abstract

Bacterial extracellular polymeric substances (EPS) contribute to the structure, surface charge, and settling properties of activated sludge by assisting in the development of bioflocs. From various soil sources, a total of twenty bacterial isolates were collected. The maximum bacterial isolate was (HS9), which produced (5.8 g/l) after being examined for exopolysaccharide (EPS) synthesis. As a result, EPSs were selected based on Bio-flocculant activity, while HS9 was the most active (86.95 %). The lowest was HS1, while the highest was HS2 (14.55 %). The synthesis and production of EPS are influenced by a variety of variables. As a result, by examining the effects of various factors on the production of EPSs by isolate (HS9), it was discovered that the maximum yield of EPS was (7.9 g/l) and cell dry was (4.1 g/l) when incubation temperature was 40 °C after three days, RPM 120, pH 7, peptone as a nitrogen source, and sucrose (20 g/l) as a carbon source.

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