Genetic variation of Pectinophora gossypiella (Saunders) treated with some insecticides using polymerase chain reaction (PCR) technique

Document Type : Original Research Papers

Authors

1 Syngenta AG Research Station

2 Plant Protection Dep., Faculty of Agriculture, Benha Uni., Moshtohor , Qalubia, 13736, Egypt

3 Plant Protection Research Institute

Abstract

Random Amplified Polymorphic DNA (RAPD) was used to examine the pooled genomic DNA of Pectinophora gossypiella 4th instar larvae treated with recommended doses of various pesticides in the field strain of the pink bollworm (Pectinophora gossypiella). Insecticide-free cotton fields were used to collect a field strain of the pest. Four insecticides utilized in this study agristar, proclaim, tracer, and Camkron. A total of five primers were employed to examine the mutagenic differences between the treated strains. Most fragments were created with the HB-12 primer (10 fragments). Primers HB-11 and HB-12 have the fewest pieces, respectively (6 fragments). A total of seven to nine overlapping fragments were produced by the other three primers. Fragments varied in size from 180 to 1435 bp in molecular weight. One of the pink bollworm P. gossypiella 4th instar larvae treated with proclaim insecticide had a lower similarity index (0.38) than the untreated one, indicating the greatest degree of change in DNA structure and sequences between untreated pink bollworm larvae and those treated with various insecticides. The RAPD patterns that resulted from this comparison showed that Primers HB-11, HB-14, and HB-10 had the greatest similarity index (1.00) between treated populations and the control of the pink bollworm.

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Benha Journal of Applied Sciences
Volume 7, Issue 4
April 2022
Pages 283-291

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