Molecular validation for impossible transmission of TYLCV through mechanical route

Document Type : Original Research Papers

Authors

1 Microbiology and Botany, Science,Benha University, Benha, Egypt

2 Microbiology and Botany, Science , Benha University, Benha Egypt

3 Plant Genetic Transformation Department, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC).

4 Botany and Microbiology department, Faculty of Science, Helwan University, Cairo, Egypt. Postcode: 11795

Abstract

Tomato yellow leaf curl disease (TYLCD) is a serious constraint in tomato crop worldwide, affecting the production of many crops, resulting in significant economic losses. In Egypt, the incidence of infection is very high, with visual estimates of TYLCV infection reaching 100 % in some areas. Here in the mechanical transmission ability of TYLCV is assessed in different plant species well known to be hosts for the TYLCV. The current finding revealed the failness of transmission through mechanical route. None of the mechanically infected plants developed TYLC disease symptoms. Furthermore, the absence of TYLCV genome within mechanically transmitted plants was also validated. The sequence alignment of the partial TYLCV genome amplified from the naturally infected plants from Qalyubia governorate, show 98.99% similarity and 100% query coverage to the TYLCV in the GenBank. In conclusion, the TYLCV can’t transmit through mechanical route this may be a result of failing its genome to be establish within plant genome.

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