Effect of different parameters on the production of Exopolysaccharides with Antioxidant activity from marine Streptomycetes

Document Type : Original Research Papers

Authors

1 Microbial Biotechnology Dept., Division of Biotechnology Research Division, National Research Centre

2 Botany and Microbiology Dept., Faculty of science, Benha University, Egypt.

Abstract

Marine bacteria have amazing ways of adapting to harsh environments. One way they do this is by making a wide range of bioactive compounds that have different metabolic and physiological properties. These adaptations ensure their resilience in severe conditions and facilitate their significant contributions to the oceanic ecosystem. Hence, it is anticipated that the bacteria obtained from marine environments will exhibit enhanced efficacy in the synthesis of novel bioactive chemicals. A total of twenty-two streptomycetes isolates were collected from diverse marine samples sourced from Hurghada, Safaga, and Marsa Alam. The isolates were assessed for their capacity to generate exopolysaccharides (EPSs). As a result, isolate (S10) was chosen due to its exceptional productivity, reaching a maximum of 7.6 g/l. So, the antioxidant potential of EPSs was measured by how well they got rid of DPPH free radicals. The highest score achieved at the 120-minute mark was EPSS10, with a mean value of 88.74% ± 1.1%. Conversely, the synthesis and production of EPSs are subject to several circumstances. Hence, the impact of these variables on the formation of exopolysaccharides (EPSs) by the isolate (S10) was observed. The findings indicated that the highest EPS yield recorded was 8.5 g/l, achieved under the following conditions: incubation temperature of 30°C for a duration of six days, rotational speed of 150 rpm, pH level of 7, utilization of peptone as the nitrogen source, and utilization of starch as the carbon source.

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Benha Journal of Applied Sciences
Volume 8, Issue 12
December 2023
Pages 23-30

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