Silica Nanoparticles Biosynthesis from Saccharomyces sp. with antimicrobial activity

Document Type : Original Research Papers

Authors

1 قسم النبات شعبة ميكروبيولوجي ـــ كلية العلوم ـــ جامعة بنهاــ مدينة بنها ـــ مصر

2 Botany and Microbiology Department, Faculty of Science, Benha University, Egypt

3 Microbial Chemistry department , National Research Centre , El Buhouth St. 33, Cairo 12622, Egypt

4 Professor of Botany and Microbiology Department , Faculty of Science, Benha University

5 Main laboratories for Egyptian Army

Abstract

The present study focuses on the isolation of Saccharomyces spp. from bakery yeast samples and their potential for biosynthesizing silica nanoparticles (SiNPs) with antibacterial properties. Yeast samples were collected from four different bakeries in Benha to ensure strain diversity, and multiple isolates were successfully obtained. Screening for silica nanoparticle biosynthesis revealed significant variations among the isolates, with DO1S4 and DO4S1 exhibiting the highest biosynthesis potential (+++), indicating strong enzymatic activity and effective interaction with silica precursors. Moderate biosynthesis (++) was observed in isolates DO1S1 and DO2S3, while several isolates showed weak or no biosynthesis activity. The antibacterial activity of SiNPs synthesized from the highly active strain DO1S4 was evaluated against Escherichia coli and Staphylococcus aureus. The biosynthesized SiNPs demonstrated significant antibacterial effects, with inhibition rates of 77.025% against E. coli and 52.02% against S. aureus. However, their efficacy was lower than ciprofloxacin (5 µg/mL), which exhibited inhibition rates of 99.05% and 98.25%, respectively. The enhanced effect against E. coli compared to S. aureus is likely due to differences in bacterial cell wall structures, affecting nanoparticle penetration and antimicrobial efficiency. The antibacterial activity of the biosynthesized SiNPs is attributed to mechanisms such as reactive oxygen species (ROS) generation,

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Benha Journal of Applied Sciences
Volume 10, Issue 2 - Serial Number 2
Applied and Basic Sciences:
February 2025
Pages 17-27

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