Screening and characterization of potential probiotic lactic acid bacteria isolated from traditional dairy products

Document Type : Original Research Papers

Authors

1 Botany and Microbiology Department, Faculty of Science, Benha,13518, Egypt.

2 National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, 11878, Egypt

3 Professor of Botany and Microbiology Department , Faculty of Science, Benha University

Abstract

Lactic acid bacteria (LAB) have been used as probiotic agents due to their ability to produce a variety of beneficial compounds for cultivars and their status as safe microorganisms. Discovering and evaluating new probiotics in dairy products and other normal habitats poses a global economic and health importance. Therefore, in our study, a total of 43 traditional dairy product samples were collected from Menoufia and Qalyubia governorates for isolation of lactic acid bacteria with probiotic characteristics. Seventy-nine bacterial isolates were obtained and identified morphologically and biochemically. Among these isolates, only 23 were found to have LAB characteristics as catalase‐negative, gram‐positive and non-motile. The most potent five isolates (P18, P19, P28, P41, and P42) were tested for their probiotic characteristics, including low pH tolerance and bile salt stability. They showed high tolerance against pH 3.0 and 0.3 % bile salt concentration. Thus, this research provides a foundation for developing dairy products that can positively affect human health by incorporating probiotic microorganisms.

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Benha Journal of Applied Sciences
Volume 10, Issue 2 - Serial Number 2
Applied and Basic Sciences:
February 2025
Pages 51-59

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