MOLECULAR GENETIC ASSESSMENTOF SOME POTAO (Solanum Tuberosum) CULTIVARS

Document Type : Original Research Papers

Authors

1 Department of Genetics and Genetic Engineering, Faculty of Agriculture, Benha University

2 Genetics and Genetic Engineering Dept., Faculty of Agriculture, Benha University, Qalyubia 13736, Egypt

10.21608/bjas.2025.373870.1704

Abstract

This study performed in the Department of Genetic, Faculty of Agriculture Banha University, Egypt. Five pomegranate cultivars grown in Egypt were collected from Dakahlia Agriculture Development Company to be assessment the molecular genetic variability among five cultivars of Potato Solanum Tuberosum using SCOT and ISSR analysis. Six SCoT and five ISSR primers were tested under this study. Eighteen bands as a total number with molecular sizes ranged from 200 to 930 bp were generated from six primers of SCoT. The results obtained 9 total polymorphic bands with polymorphic percentage of (50%). While, Five ISSR primers were illustrated 22 bands with molecular sizes ranged from 230 to 1340 bp. The results obtained 10 total polymorphic bands were generated from five ISSR primers with polymorphic percentage of (45.45%). On the other hand, combination data of SCOT and ISSR primers revealed a sum of 40 band. These bands were identified as 21 monomorphic and 19 polymorphic ones with polymorphic % (47.5%) and fourteen unique markers.

The genetic similarity among tested cultivars based on combined

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