Assessment of Trial to Use Exosomes and Salmon DNA with Minoxidil as Multisource Mixture for Hair Follicle Activation an In Vivo Study

Document Type : Original Research Papers

Authors

1 1 Scientific Department, Polygon Technologies, Cairo, Egypt 2 Ahmed Maher Teaching Hospital, Cairo, Egypt

2 1 Scientific Department, Polygon Technologies, Cairo, Egypt 3 Agricultural Genetic Engineering Research Institute, Al-Giza, Egypt

3 1 Scientific Department, Polygon Technologies, Cairo, Egypt

10.21608/bjas.2025.371066.1652

Abstract

Hair loss considered the problem affecting on approximately 66% of men aged 35, while approximately 50% of women encounter noticeable hair loss during their lifetime. This study aims to evaluate the use of a mixture of multiple natural sources in addition to a commonly used drug (Minoxidil) and test its effect on modulate hair growth processes in vivo. Exosomes were isolated from human cord blood-derived mesenchymal stem cells (MSCs). Exosome characterization was performed using a Zetasizer Nano. Seven groups of C57BL/6 mice were employed as an in vivo model. Experimental groups received the following topical treatments for 10 consecutive days. Histological analysis was conducted using hematoxylin and eosin staining to evaluate hair follicle density, morphology, and distribution. Quantitative analysis of hair follicle number and size was performed using image analysis software. Furthermore, quantitative real-time polymerase chain reaction (qRT-PCR) was employed to assess the relative gene expression levels of Wnt11 and LHX2 genes. Histopathology of skin samples demonstrated a significant increase in hair follicle density and elongation in groups treated with minoxidil, with a further moderate enhancement observed in groups receiving combined treatments of exosomes and DNA polynucleotides. Quantitative qRT-PCR analysis of hair follicle tissue revealed a relative down regulation of Wnt 11 and LHX2 genes associated with hair growth inhibition in the minoxidil-treated group. Conversely, the groups treated with exosomes and DNA polynucleotides exhibited a substantial upregulation of genes associated with hair follicle proliferation and differentiation, indicating a potential mechanism for the observed hair growth stimulation.

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Benha Journal of Applied Sciences
Volume 10, Issue 2 - Serial Number 2
Applied and Basic Sciences:
February 2025
Pages 81-89

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